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- Title
The mariner Mos1 transposase produced in tobacco is active in vitro.
- Authors
Thomas, Xavier; Hedhili, Sabah; Beuf, Laurent; Demattéi, Marie-Véronique; Laparra, Hélène; Khong, Giang Ngan; Breitler, Jean-Christophe; Montandon, Frédéric; Carnus, Elodie; Norre, Frédéric; Burtin, Daniel; Gantet, Pascal; Bigot, Yves; Renault, Sylvaine
- Abstract
The mariner-like transposon Mos1 is used for insertional mutagenesis and transgenesis in different animals (insects, nematodes), but has never been used in plants. In this paper, the transposition activity of Mos1 was tested in Nicotiana tabacum, but no transposition event was detected. In an attempt to understand the absence of in planta transposition, Mos1 transposase (MOS1) was produced and purified from transgenic tobacco (HM NtMOS1). HM NtMOS1 was able to perform all transposition reaction steps in vitro: binding to ITR, excision and integration of the same pseudo-transposon used in in planta transposition assays. The in vitro transposition reaction was not inhibited by tobacco nuclear proteins, and did not depend on the temperature used for plant growth. Several hypotheses are proposed that could explain the inhibition of HM NtMOS1 activity in planta.
- Subjects
TRANSPOSONS; MOBILE genetic elements; CHROMOSOMAL translocation; PLANT genetics; TOBACCO; SMOKABLE plants
- Publication
Genetica, 2010, Vol 138, Issue 5, p519
- ISSN
0016-6707
- Publication type
Article
- DOI
10.1007/s10709-009-9414-7