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- Title
Cloning and Characterization of phzR Gene from Pseudomonas aeruginosa.
- Authors
Pang, Bingbing; Liu, Tingting; Zhang, Wenjia; Ye, Fengcai; Shang, Changhua
- Abstract
A gene encoding phzR was isolated from a phenazine-producing bacterium Pseudomonas aeruginosa 2016NX1. This paper provided the full-length cDNA encoding phzR (GenBank Accession no., MW143078). The cDNA of the phzR contained an open reading frame (ORF) of 714 bp. The potential regulatory elements were predicted in the phzR promoter region. The deduced amino acid sequence of P. aeruginosa phzR showed significant homology to the known phzRs from different organisms. Gene overexpression analysis showed that the phenazine content was improved (44.39%) in comparison to wild-type 2016NX1.
- Subjects
MOLECULAR cloning; PSEUDOMONAS aeruginosa infections; AMINO acid sequence; GENETIC overexpression; COMPLEMENTARY DNA; GENES; PROMOTERS (Genetics); PSEUDOMONAS
- Publication
Current Microbiology, 2021, Vol 78, Issue 4, p1482
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-021-02410-2