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- Title
Protective immunity against Eimeria tenella infection in chickens following oral immunization with Bacillus subtilis expressing Eimeria tenella 3-1E protein.
- Authors
Lin, Zhiwei; Shi, Yanyun; Deng, Bin; Mao, Xiangfei; Yu, Dongyou; Li, Weifen
- Abstract
The current experiment was conducted to construct recombinant Bacillus subtilis WB600 expressing Eimeria tenella 3-1E protein to investigate the oral immunization protective effects against E. tenella. The merozoite surface antigen 3-1E gene of E. tenella was introduced into the pBS- H1 expression vector with a novel signal peptide sequence. After the electro-transformation, the expression of objective protein in B. subtilis WB600 was detected by Western blot. The results showed that the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Seven-day-old broiler chickens were orally vaccinated with B. subtilis WB600 harboring 3-1E ( B.S- pBS- H1- 3- 1E) or B. subtilis WB600 with empty plasmid ( B.S- pBS- H1) 10 days prior to challenge with sporulated E. tenella oocysts. The results showed the recombinant B. subtilis strain with the ability of high-level secretion of 3-1E was constructed successfully. Vaccination with B.S- pBS- H1- 3- 1E strain significantly increased the anti-coccidial index and reduced cecal lesion scores compared with the positive control group (chickens were challenged with sporulated E. tenella oocysts without oral administration of B.S- pBS- H1- 3- 1E strain) and B.S- pBS- H1 group. Ceca mucosal sIgA, secretion, and IL-2, IL-12, IFN-γ, and IL-10 level after challenge were greater in the B.S- pBS- H1- 3- 1E group than in the positive control group. Taken together, these results indicated that B. subtilis WB600 harboring 3-1E protein induces protective immunity against E. tenella.
- Subjects
BACILLUS subtilis genetics; EIMERIA tenella; BACTERIAL disease risk factors; CHICKEN diseases; SIGNAL peptides; IMMUNIZATION
- Publication
Parasitology Research, 2015, Vol 114, Issue 9, p3229
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-015-4539-3