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- Title
Characterization of neuron-like cells derived from canine bone marrow stromal cells.
- Authors
Oda, Yasutaka; Tani, Kenji; Kanei, Toshitaka; Haraguchi, Tomoya; Itamoto, Kazuhito; Nakazawa, Hiroshi; Taura, Yasuho
- Abstract
Regenerative therapy using bone marrow stromal cells (BMSCs) has begun to be clinically applied in humans and dogs for neurological disorders such as spinal cord injury. Under appropriate conditions in vitro, BMSCs differentiate into neuronal cells, which may improve the effects of regenerative therapy. In this study, we evaluated canine neuron-like cells (NLCs) derived from BMSCs. We speculated on their suitability for neuro-transplantation from the point of view of their morphological features, long-term viability, abundant availability, and ability to be subcultured. Canine NLCs were differentiated as follows: third-passage BMSCs were maintained in pre-induction medium containing 2-mercaptoethanol and dimethylsulfoxide for 5 h, and then cells were transferred to neuronal induction medium containing fetal bovine serum, basic fibroblast growth factor, epidermal growth factor, dibutyryl cyclic AMP, and isobutylmethylxanthine for 7 or 14 days. Canine NLCs fulfilled the transplantation criteria and expressed markers of both immature neurons (nestin, 84.7 %) and mature neuronal cells (microtubule-associated protein-2, 95.7 %; βIII-tubulin protein, 12.9 %; glial fibrillary acidic protein, 9.2 %). These results suggest that canine BMSCs can be induced to differentiate into neuronal cells and may be suitable for neuro-transplantation. This study may provide information for improving cellular therapy for neurological diseases.
- Subjects
MESENCHYMAL stem cells; CELL transplantation; CELL morphology; MERCAPTOETHANOL; DIMETHYL sulfoxide; FIBROBLAST growth factors; EPIDERMAL growth factor; NEURONS
- Publication
Veterinary Research Communications, 2013, Vol 37, Issue 2, p133
- ISSN
0165-7380
- Publication type
Article
- DOI
10.1007/s11259-013-9555-0