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- Title
Optimization of Structural and Optical Parameters of Plasmon Silver Films for IgG-FITC Fluorescent Analysis.
- Authors
Kulakovich, O. S.; Shcherbovich, A. A.; Ramanenka, A. A.; Koktysh, I. V.; Melnikova, Ya.I.; Gaponenko, S. V.; Maskevich, S. A.
- Abstract
The fluorescence enhancement factor of the conjugate of immunoglobulin G labeled with fluorescein isothiocyanate (IgG-FITC) as an immunofluorescent marker was optimized as a function of the optical and topographic parameters of a colloidal silver film on the surface of a standard polystyrene immunoassay plate. The factors influencing fluorescence enhancement were identified. The fluorescence enhancement was shown to be a plasmon-resonance process by using time-resolved spectroscopy, analysis of fluorescence excitation spectra, and enzyme immunoassay determination of the relative concentrations of IgG-FITC adsorbed on the solid phase. The optical density of the silver colloidal film at the excitation and emission wavelengths of IgG-FITC was the most important parameter correlated with the fluorescence enhancement factor. The maximum enhancement factor of 10.2 times was obtained for silver films with the highest optical density.
- Subjects
STRUCTURAL optimization; COLLOIDAL silver; OPACITY (Optics); EXCITATION spectrum; IMMUNOGLOBULIN G; FLUORESCENCE spectroscopy; TIME-resolved spectroscopy
- Publication
Journal of Applied Spectroscopy, 2023, Vol 90, Issue 1, p42
- ISSN
0021-9037
- Publication type
Article
- DOI
10.1007/s10812-023-01500-2