We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Chemical analysis and giardicidal effectiveness of the aqueous extract of <italic>Cymbopogon citratus</italic> Stapf.
- Authors
Méabed, Eman M. H.; Abou-Sreea, Alaa I. B.; Roby, Mohamed H. H.
- Abstract
Searching for new effective and safe treatment of <italic>Giardia lamblia</italic> (<italic>G. lamblia</italic>) parasite is mandatory. The aim was to evaluate the in vitro and in vivo effectiveness of an aqueous extract prepared from the leaves of <italic>Cymbagogon citratus</italic> (CcAE) against <italic>G. lamblia</italic> and to reveal the phenolic and antioxidant properties of CcAE. Methods: CcAE (25, 50, 100, 200, 400, and 500 μg/ml) was in vitro incubated with <italic>G. lamblia</italic> trophozoites in comparison with metronidazole (MTZ 10 and 25 μg/ml). Growth inhibition was evaluated after 3, 24, and 48 h of drug exposure. Infected groups of mice were orally treated for 7 days with CcAE at 125, 250, and 500 mg/kg/day/mouse, in comparison with a group treated with 15 mg/kg/day/mouse MTZ for the same period. The total phenolic components (TPC), the total flavonoid components (TFC), the 2,2,diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and the high-performance liquid chromatography (HPLC) for quantitative and qualitative phenolic content were chemically estimated. After 24 and 48 h of in vitro incubation, the estimated minimal inhibitory concentrations (MIC) were 500 and 400 μg/ml, respectively, and the concentrations that induced 50% growth inhibition (IC50) were 93.8 and 60.4 μg/ml, respectively (<italic>P</italic> < 0.001). Mice given 500 mg/kg CcAE showed 100% stool clearance of <italic>G. lamblia</italic> stages, similar to MTZ-treated control group (<italic>P</italic> < 0.001). The TPC was 10.7 ± 0.2 mg GAE/g and the TFC was 23.9 ± 0.3 mg quercetin/g, and the estimated IC50 for DPPH free radical scavenging was 16.4 ± 0.1 mg/ml. HPLC revealed the major phenolic components of CcAE to be carnosic acid, <italic>p</italic>-coumaric acid, cinnamiac acid, quercetin, rutin, and chlorogenic acid. In conclusion, CcAE is significantly effective against <italic>G. lamblia</italic> in vitro and in vivo, and has considerable phenolic and antioxidant properties.
- Subjects
GIARDIA lamblia; LEMONGRASS; METRONIDAZOLE; GIARDIASIS; BENZIMIDAZOLES; NITROIMIDAZOLES; DISEASE risk factors; THERAPEUTICS
- Publication
Parasitology Research, 2018, Vol 117, Issue 6, p1745
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-018-5855-1