We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection of the plasmid-encoded fosfomycin resistance gene fosA3 in Escherichia coli of food-animal origin.
- Authors
Hou, Jianxia; Yang, Xiaoyun; Zeng, Zhenling; Lv, Luchao; Yang, Tong; Lin, Dachuan; Liu, Jian-Hua
- Abstract
Objectives To investigate the occurrence of plasmid-mediated fosfomycin resistance genes among Escherichia coli from food animals in China. Methods A total of 892 E. coli isolates collected from individual pigs (n = 368), chickens (n = 196), ducks (n = 261), geese (n = 35), pigeons (n = 20) and partridges (n = 12) in Guangdong Province during 2002–08 were screened for the presence of fosA3, fosA and fosC2 by PCR amplification and sequencing. The clonal relationship of fosA3-positive isolates, plasmid content and other associated resistance genes were also characterized. Results Twelve (1.3%) E. coli isolates showed resistance to fosfomycin and 10 (1.1%) isolates (4 from pigs, 2 from chickens, 2 from ducks, 1 from a goose and 1 from a pigeon) were positive for fosA3. None of the E. coli isolates was positive for fosA or fosC2. All of the isolates carrying fosA3 were CTX-M producers, and three of them carried rmtB. Most of the fosA3-harbouring isolates were found to be clonally unrelated. The fosA3 genes were flanked by IS26. Two fosA3 genes co-localized with rmtB and blaCTX-M-65 on indistinguishable F33:A-:B- plasmids that carried three addiction systems (pemI/pemK, hok/mok/sok and srnB). Four, one and one fosA3 genes were found to be associated with IncN (ST8 type), IncI1 and F2:A-:B- plasmids, respectively. Conclusions We discovered that fosA3 is always associated with blaCTX-M, which facilitates its quick dispersal. The emergence of fosA3 in food animals could impact on human medicine by the potential transfer of resistance through the food chain.
- Subjects
GUANGDONG Sheng (China); PLASMID genetics; FOSFOMYCIN; ESCHERICHIA coli; FOOD animals; DISEASES
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2013, Vol 68, Issue 4, p766
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dks465