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- Title
Effect of Cultivation Conditions on the Growth and Activities of Sulfur Metabolism Enzymes and Carboxylases of Sulfobacillus thermosulfidooxidans subsp. asporogenes Strain 41.
- Authors
Egorova, M. A.; Tsaplina, I. A.; Zakharchuk, L. M.; Bogdanova, T. I.; Krasil'nikova, E. N.
- Abstract
The moderately thermophilic acidophilic bacterium Sulfobacillus thermosulfidooxidans subsp. asporogenes strain 41 is capable of utilizing sulfides of gold–arsenic concentrate and elemental sulfur as a source of energy. Growth in the presence of S0 under auto- or mixotrophic conditions was less stable than in media containing iron monoxide. The enzymes involved in the oxidation of sulfur inorganic compounds—thiosulfate-oxidizing enzyme, tetrathionate hydrolase, rhodanase, adenylyl phosphosulfate reductase, sulfite oxidase, and sulfur oxygenase—were determined in the cells of the sulfobacilli grown in mineral medium containing 0.02% yeast extract and either sulfur or iron monoxide and thiosulfate. Cell-free extracts of the cultures grown in the medium with sulfur under auto- or mixotrophic conditions displayed activity of the key enzyme of the Calvin cycle—ribulose bisphosphate carboxylase—and several other enzymes involved in the heterotrophic fixation of carbon dioxide. Activities of carboxylases depended on the composition of the cultivation media.
- Subjects
TILLAGE; SULFUR in soils; BACTERIAL growth; METALLOENZYMES; ARSENIC; OXIDATION; INORGANIC compounds; SOIL microbiology
- Publication
Applied Biochemistry & Microbiology, 2004, Vol 40, Issue 4, p381
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1023/B:ABIM.0000033915.97286.0e