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- Title
Highly Antifungal Activity of Biosynthesized Copper Oxide Nanoparticles against Candida albicans.
- Authors
Garcia-Marin, Luis Enrique; Juarez-Moreno, Karla; Vilchis-Nestor, Alfredo Rafael; Castro-Longoria, Ernestina
- Abstract
Candida albicans (ATCC SC5314) was exposed to biosynthesized copper oxide nanoparticles (CuONPs) to determine their inhibitory capacity. Nanoparticles were polydisperse of small size (5.8 ± 3.5 nm) with irregular shape. The minimum inhibitory concentration (MIC) against C. albicans was 35.5 µg/mL. The production of reactive oxygen species (ROS) of C. albicans was verified when exposed to different concentrations of CuONPs. Ultrastructural analysis of C. albicans revealed a high concentration of CuONPs in the cytoplasm and outside the cell; also, nanoparticles were detected within the cell wall. Cytotoxic analyses using fibroblasts (L929), macrophages (RAW 264.7), and breast (MCF-12) cell lines show good results of cell viability when exposed at the MIC. Additionally, a hemocompatibility analysis was carried out and was found to be below 5%, considered the threshold for biocompatibility. Therefore, it is concluded that the biosynthesized CuONPs have a high potential for developing a topical antifungal treatment.
- Subjects
CANDIDA albicans; COPPER oxide; NANOPARTICLES; REACTIVE oxygen species; CELL survival; CELL lines
- Publication
Nanomaterials (2079-4991), 2022, Vol 12, Issue 21, p3856
- ISSN
2079-4991
- Publication type
Article
- DOI
10.3390/nano12213856