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- Title
Evaluation of Antimicrobial Activity and Cytotoxicity Effects of Extracts of Piper nigrum L. and Piperine.
- Authors
Alves, Fabrine Silva; Cruz, Jorddy Neves; de Farias Ramos, Ingryd Nayara; do Nascimento Brandão, Dayse Lucia; Queiroz, Rafael Nascimento; da Silva, Glauce Vasconcelos; da Silva, Gleice Vasconcelos; Dolabela, Maria Fani; da Costa, Marcondes Lima; Khayat, André Salim; de Arimatéia Rodrigues do Rego, José; do Socorro Barros Brasil, Davi
- Abstract
P. nigrum L. extracts and the piperine alkaloid have important antimicrobial, anti-inflammatory, and antioxidant properties. Therefore, in this study, we evaluated the antimicrobial activity and cytotoxicity of P. nigrum L. extracts and piperine, a compound isolated from the extracts of P. nigrum L. Extracts obtained via maceration, soxhlet, and purification steps, in addition to isolated piperine, were used in this study. Spectroscopic methods, such as nuclear magnetic resonance, scanning electron microscopy, X-ray diffraction, thermogravimetry, and differential scanning calorimetry, were used to characterize piperine. In the microbiological analyses, the extract obtained via maceration-derived sample showed high efficiency in inhibiting Salmonella spp. (MIC < 100 μg/mL). The extract obtained via a soxhlet-derived sample showed promising inhibitory activity against almost all microorganisms, with negligible inhibition of Pseudomonas aeruginosa. Favorable inhibition coefficients were also observed against Staphylococcus aureus and Salmonella spp. (MIC < 100 μg/mL) for the extract obtained via purification of the steps-derived sample. Piperine showed an excellent inhibition coefficient against most microorganisms, with inactivity only observed against P. aeruginosa. Cytotoxicity evaluation assays in cancer cell lines revealed that piperine exhibited inhibitory potential on all tested tumor cell lines, causing a decrease in cell viability and achieving an IC50 of less than 30 μg/mL. The analyzed extracts from P. nigrum L. seeds showed cytotoxic activity against tumor and non-tumor cell lines.
- Subjects
BLACK pepper (Plant); ANTI-infective agents; NUCLEAR magnetic resonance; DIFFERENTIAL scanning calorimetry; SCANNING electron microscopy; EXTRACTS; SALMONELLA
- Publication
Separations (2297-8739), 2023, Vol 10, Issue 1, p21
- ISSN
2297-8739
- Publication type
Article
- DOI
10.3390/separations10010021