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- Title
Design and characterization of a bi-functional bybrid antibacterial peptide LLM against Pseudomonas aeruginosa.
- Authors
ZHANG Ze‑jin; YAO Hong‑ji; XUE Zhao‑yi; ZHAN Yu‑tong; GUO Ying‑jie; WU Meng; LIN Ke‑xin; XIONG Jing‑yan; ZHANG Yong
- Abstract
Objective: To design a bifunctional antimicrobial peptide with high antibacterial activity and endotoxin neutralization against P.aeruginosa and explore its bactericidal properties. Methods: The neutralizing endotoxin peptides and antimicrobial peptide against P.aeruginosa were connected by a GGGS-linker to get a bi-functional bybrid peptide. Its structural parameter was tested by EMBOSS software. The minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and bactericidal kinetics against P.aeruginosa were determined. Its effect on endotoxin neutralization and hemolysis were also evaluated. Results: We designed and obtained a bybrid peptide LLM, which carried +8 positive charge with high activity against P.aeruginosa and neutralizing endotoxin. The MIC of LLM against P.aeruginosa CMCC10104 and P.aeruginosa ATCC 9027 were 2 and 4 μmol/L, and MBC/MIC equal 1. LLM showed rapid anti- P.aeruginosa effects and significantly neutralized the endotoxin released, and not exhibited hemolysis as high as 115 μmol/L (400 μg/mL).Conclusion: The MICs of LLM against P.aeruginosa were 2~4 μmol/L, it showed significant activity of anti-P. aeruginosa and neutralizing endotoxin, and could kill bacteria quickly,and did not show significant hemolytic under 115 μmol/L.
- Subjects
PEPTIDE antibiotics; PEPTIDES; PSEUDOMONAS aeruginosa; ANTIMICROBIAL peptides; ENDOTOXINS; ANTIBACTERIAL agents
- Publication
Journal of Hainan Medical University, 2022, Vol 28, Issue 21, p27
- ISSN
1007-1237
- Publication type
Article