We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
THE SELECTION OF TECHNOLOGICAL PARAMETERS IN ORDER TO OBTAIN AN EXTRACT WITH IMPORTANT ANTIOXIDANT ACTIVITY FROM STINGING NETTLE LEAVES.
- Authors
NENCU, IOANA; POPESCU, LIDIA MARIA; ISTUDOR, VIORICA; COSTEA, TEODORA; DUŢU, LIGIA ELENA; GÎRD, CERASELA ELENA
- Abstract
The aim of this study was to examine the effects of the plant stage development and the extraction parameters (solvent polarity and extraction procedure) on stinging nettle polyphenolic content and antioxidant activity, in order to select the optimal technological parameters for the obtaining of an extract with high antioxidant activity from stinging nettle leaves. The total polyphenolic (T.P.) content of the extracts was determined using Folin Ciocalteu's method. The antioxidant potential was assessed using the DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS [2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid] methods. The extract with the highest antioxidant effect was obtained by reflux extraction of leaves with 50% ethanol (water:ethanol = 50:50, v/v) (extract D 37.0 mg% tannic acid, IC500 for DPPH and ABTS are 50.39 μg/mL, respectively 91 μg/mL). The correlation factors of ABTS-T.P. and DPPH-T.P. were high for the immature leaves (0.94 and 0.96, respectively) and modest for mature ones (0.76 and 0.33). These results imply that T.P. are major contributors to the antioxidant activity of young nettle leaves. In conclusion, our research indicated that the optimal technological parameters for obtaining extracts with high antioxidant activity from stinging nettle leaves are: refluxing immature raw material using 50% ethanolic solution.
- Subjects
STINGING nettle; THERAPEUTIC use of plant extracts; ANTIOXIDANTS; PLANT development; CHEMICAL composition of plants; THERAPEUTICS
- Publication
Farmacia, 2017, Vol 65, Issue 2, p295
- ISSN
0014-8237
- Publication type
Article