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- Title
Caloric restriction results in decreased expression of peroxisome proliferator-activated receptor superfamily in muscle of normal and long-lived growth hormone receptor/binding protein knockout mice.
- Authors
Masternak, Michal M.; Al-Regaiey, Khalid A.; Del Rosario Lim, Marc Michael; Bonkowski, Michael S.; Panici, Jacob A.; Przybylski, Grzegorz K.; Bartke, Andrzej; Smith, James R.
- Abstract
Resistance to growth hormone, reduced insulin-like growth factor 1 (IGF1) action, and enhanced insulin sensitivity are likely mediators of extended life span and delayed aging process in growth hormone receptor/binding protein knockout (GHR-KO) mice. Fat metabolism and genes involved in fatty acid oxidation are strongly involved in insulin action. Using real-time polymerase chain reaction and western blot we have examined expression of peroxisome proliferator-activated receptors (PPARs) and retinoid X receptor (RXR) genes in the skeletal muscle of normal and GHR-KO mice subjected to 30% caloric restriction. The results indicate that caloric restriction decreased the expression of PPARgamma, PPARalpha, and PPARbeta/delta which would lead to down-regulation of fat metabolism. This suggested metabolic change clearly does not affect whole-body insulin action. These findings suggest that whole-animal insulin sensitivity is not regulated through skeletal muscle insulin action.
- Subjects
SOMATOTROPIN; SOMATOMEDIN; LIFE spans; AGING; CARRIER proteins; LABORATORY mice; METABOLISM; GENES; FATTY acids; OXIDATION; INSULIN; PEROXISOMES
- Publication
Journals of Gerontology Series A: Biological Sciences & Medical Sciences, 2005, Vol 60, Issue 10, p1238
- ISSN
1079-5006
- Publication type
journal article
- DOI
10.1093/gerona/60.10.1238