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- Title
Gene expression analysis of sugarcane response to water stress based on cDNA-SCoT differential display.
- Authors
WU Kai-chao; HUANG Cheng-mei; DENG Zhi-nian; WEI Yuan-wen; CAO Hui-qing; XU Lin; JIANG Sheng-li; WU Jian-ming; YANG Li-tao; LI Yang-rui
- Abstract
[Objective] The present study was conducted to study the expression of differential genes in sugarcane response to water stress and its molecular mechanism in order to provide scientific basis for developing genetic improvement of sugarcane drought resistant integrity. [ Method] Sugarcane cultivar GT11 was used as material to design three treatments viz. control, drought and rewatering. The +1st leaves of sugarcane for three treatments were used for extracting total RNA to construct their equal quantity mixed solutions, and the genetic expressing profiles of cultivar GT11 at elongation stage under water stress were constructed by using cDNA-SCoT differential display. The transcript derived fragments(TDFs) were screened and isolated to sequencing, followed by the BLAST homology searching in NCBI database to deduce gene function based on homological genes. [Result] The 180 TDFs were screened by using 42 primers of SCoT marker, of which 100 TDFs were of higher similarity with the accessed genes in NCBI database. According to homologous gene function, these 100 TDFs could be classified into 14 types, which included 11 metabolism related genes, 12 energy metabolism related genes, 5 transport pathway related genes, 6 communication and signal transduction related genes, 3 cell cycle and DNA processing related genes, 3 transcript regulated factor related genes, 17 protein synthesis related genes, 1 protein processing related genes, 7 associated-functional protein related genes, 3 interaction with the environment related genes, 5 transposons, toxicity and plasmid protein related genes, 1 cell component biological synthesis related genes, 4 defense related genes, and 22 un-functional protein related genes. [Conclusion] The regulating pathways of sugarcane response to water stress are of multiformity, complexity, coordination and networked; and the obtained water stress-related TDFs by cDNA-SCoT differential display could provide important information to further explore molecular mechanism sugarcane response to water regulation pathway.
- Subjects
SUGARCANE; GENE expression in plants; ANTISENSE DNA; PHYSIOLOGY
- Publication
Journal of Southern Agriculture, 2016, Vol 47, Issue 12, p1999
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j:issn.2095-1191.2016.12.1999