We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Quorum-Sensing Inhibition by Furanone Compounds and Therapeutic Effects on Pseudomonas Aeruginosa Keratitis Rabbit Model.
- Authors
DAL, Aleyna; ELBAY, Ahmet; HEKİMOĞLU, Emine Rümeysa; SÜMBÜL, Bilge; KOÇYİĞİT, Abdurrahim; BABALI BALIBEY, Fatmanur; ÖZMAN, Zeynep
- Abstract
Introduction: Keratitis is the inflammation of the cornea and associated with both infectious and noninfectious diseases. A mechanism called Quorum Sensing (QS) is the communication between bacterial cells reliant on cell density and the concentration of specific signaling molecules. Inhibitors of QS are an advanced strategy discovered to decrease P. aeruginosa pathogenesis and its virulence. Based on the previous studies and proven effects of furanones as a QS inhibitor on pseudomonas infection, this study aims to investigate QS inhibition by furanone compounds in P. aeruginosa keratitis rabbit model. Method: Thirty adults New Zealand white rabbits were used in this study. Corneas of anesthetized rabbits were intrastromally injected with bacteria. Rabbits were randomly divided into six groups. Control group only infected with P. aeruginosa. Starting 1 hour after inoculation 0.1 mg/mL (B), 0.2 mg/mL (C), 0.3 mg/mL (D) Furanone, 50 mg/mL ceftazidime (A) and 20% dimethyl sulfoxide (E) were used with one drop per hour. Rabbits were sacrificed 3 days later and corneas were collected. Results: Collected samples were evaluated clinically, histologically and biochemical. In all evaluations, the therapeutic response in the antibiotic group was better than other groups. Slit lamp examination score of Group C was significantly lowered compared to the control (p=0.009). Histological evaluation shows that inflammation is decreased in B, C, and D groups. Reactive oxygen species values were significantly lower in group B (p=0.001) and C (p=0.01). And there was no statistical significance among Superoxide dismutase values. Conclusion: The comprehensive findings demonstrate that furanone showed an anti-inflammatory effect. However, its therapeutic effect has not been observed to be sufficient compared to antibiotics. Further investigation is necessary to explore the protective effects and mechanism of furanones on pseudomona keratitis.
- Subjects
NEW Zealand; FURANONES; PSEUDOMONAS aeruginosa; KERATITIS; SLIT lamp microscopy; PSEUDOMONAS diseases; DIMETHYL sulfoxide; SUPEROXIDES
- Publication
Bezmialem Science, 2022, Vol 10, pS10
- ISSN
2148-2373
- Publication type
Abstract