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- Title
Isolation of Different Azo Dye Decolorizing Bacteria and Their Decolorization Mechanisms.
- Authors
LinLin Quan; Jiamin Huang; Jicheng Qi; Yuling Zhu
- Abstract
Four strains, which can decolorize acid orange, congo red, methyl orange and amino black respectively, were isolated from anaerobic activated sludge using the enrichment, acclimation and purification methods. With 16S rDNA sequencing analysis, they were identified as Serratia nematodiphila subsp. sakuensis st, Morganella morganii subsp. morganii str, Lysinibacillus sp. BAB-5845 and Bacillus sp. NJSN49. The decolorization conditions were studied and results demonstrated that the optimal conditions for the four strains were starch of 4 g/L, peptone of 8 g/L, pH 7.5; sucrose of 2 g/L, yeast powder of 2 g/L, pH 7.5; yeast powder of 2 g/L, pH 7.0 and sucrose of 8 g/L, urea of 4 g/L, pH 6.0. UV-Vis, FTIR and GC-MS analysis showed that, with the four strains, -N=N- double bond in acid orange, congo red, methyl orange and amino amine were broken effectively and the related aromatic amino were yielded. Our research can provide efficient strains and technical parameters for azo dye wastewater treatment industry.
- Subjects
AZO dyes; ACTIVATED sludge process; ACCLIMATIZATION; PROTEUS morganii; NUCLEOTIDE sequencing; WASTEWATER treatment
- Publication
Nature Environment & Pollution Technology, 2018, Vol 17, Issue 3, p981
- ISSN
0972-6268
- Publication type
Article