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- Title
Toll-like Receptor 2-Mediated Gene Expression in Epithelial Cells DuringHelicobacter pyloriInfection.
- Authors
Ding, Song-Ze; Torok, Anastasia M.; Smith, Michael F.; Goldberg, Joanna B.
- Abstract
Helicobacter pyloriis the major pathogen causing chronic gastritis and peptic ulcer disease and is closely linked to gastric malignancy. We have previously shown thatH. pylori-induced NF-κB activation and interleukin (IL)-8 secretion are mediated by Toll-like receptor (TLR) 2 in epithelial cells. However, the TLR2-mediated global gene expression profile of the epithelial cell duringH. pyloriinfection is still unknown. The goal of this study was to identify TLR2-regulated genes in epithelial cells induced byH. pylori.The HEK293 and HEK-TLR2 cells were cocultured withH. pylori26695 for 6 hours. Total RNA was extracted and hybridized to the Affymetrix human U133A microarray chipset, which contains 22,283 total probe sets including 14,285 genes. Data analyses were performed usingaffymetrix suite5 software. The expression of selected genes in gastric epithelial cells AGS and MKN45 was monitored by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).Forty-six genes, contained in 57 probe sets, were induced> 2-fold and three genes (five probe sets) decreased> 2-fold byH. pyloriinfection of HEK293 cells. Fifty-four genes, contained in 69 probe sets, were induced> 2-fold, whereas only 1 gene was repressed> 2-fold inH. pylori-infected HEK-TLR2 cells. Comparisons of genes induced in HEK293 or HEK-TLR2 cells identified 28 genes whose expression was dependent on the presence of TLR2. Seventeen genes were selected and their expression was assessed using the quantitative RT-PCR in gastric epithelial cells duringH. pyloriinfection.Eight of the 17 genes showed distinct expression patterns in AGS and MKN45 cells afterH. pyloristimulation.The current study investigated the TLR2-mediated global gene changes afterH. pyloristimulation in the epithelial cell system. This approach will be helpful in identifying genes whose expression is mediated by specific TLRs and in determining the cellular responses that are responsible for diverse signal pathways duringH. pyloriinfection.
- Subjects
HELICOBACTER pylori infections; HELICOBACTER diseases; GRAM-negative bacterial diseases; EPITHELIAL cells; EPITHELIUM; HELICOBACTER pylori; HELICOBACTER
- Publication
Helicobacter, 2005, Vol 10, Issue 3, p193
- ISSN
1083-4389
- Publication type
Article
- DOI
10.1111/j.1523-5378.2005.00311.x