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- Title
Inhibition of Helicobacter pylori -induced cyclo-oxygenase-2 aggravates NSAID-caused gastric damage in Mongolian gerbils.
- Authors
Futagami, S.; Hiratsuka, T.; Wada, K.; Tatsuguchi, A.; Tsukui, T.; Miyake, K.; Akamatsu, T.; Hosone, M.; Sakamoto, C.; Kobayashi, M.
- Abstract
Background: The effect of Helicobacter pylori infection on non-steroidal anti-inflammatory drug-induced gastric mucosal injury is controversial. Aim: To examine the effect of the interaction between H. pylori and non-steroidal anti-inflammatory drugs on gastric mucosal injury. Methods: Mongolian gerbils infected with H. pylori were treated with indometacin at 8 mg/kg for 2 days or 7 days. Mucosal damage was assessed by macroscopic and histological examination, and myeloperoxidase activity was measured as an index of neutrophil infiltration. The expression levels of cyclo-oxygenase proteins were determined by Western blot analysis and cyclo-oxygenase activity. Results: A 2-day course of indometacin did not cause an increase in gastric damage in H. pylori -infected Mongolian gerbils compared to uninfected gerbils, while a 7-day course of indometacin caused additive gastric damage in H. pylori -infected animals. H. pylori infection induced cyclo-oxygenase-2 expression in the stomach. Treatment with indometacin for 2 days did not significantly affect cyclo-oxygenase activity in H. pylori -infected animals, while treatment for 7 days inhibited both cyclo-oxygenase-1 and cyclo-oxygenase-2 activities. Pre-treatment with a selective cyclo-oxygenase-2 inhibitor aggravated mucosal injury in H. pylori -infected animals treated or not treated with indometacin for 2 days. Conclusions: Our results suggest that cyclo-oxygenase-2 protein induced by H. pylori infection may be involved in the defence of the gastric mucosa against damage caused by non-steroidal anti-inflammatory drugs. Therefore, inhibition of cyclo-oxygenase-2 activity may enhance non-steroidal anti-inflammatory drug-caused gastric damage in H. pylori -infected animals.
- Subjects
GASTRIC mucosa; HELICOBACTER pylori; NONSTEROIDAL anti-inflammatory agents; MONGOLIAN gerbil; WOUNDS &; injuries
- Publication
Alimentary Pharmacology & Therapeutics, 2002, Vol 16, Issue 4, p847
- ISSN
0269-2813
- Publication type
Article
- DOI
10.1046/j.1365-2036.2002.01211.x