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- Title
Initiation of HIV Reverse Transcription: Is Enzyme Flipping Required?
- Authors
Götte, Matthias
- Abstract
Liu and colleagues have recently studied dynamic changes in the orientation of HIV reverse transcriptase (RT) on its nucleic acid substrate during initiation of DNA synthesis. The authors employed a single molecule FRET assay and revealed the existence of an equilibrium between polymerase-competent and “flipped” polymerase-incompetent orientations. RT flipping correlates with enzyme pausing during initiation, while the transition to the processive elongation phase correlates with increases in the population of polymerase-competent complexes. The potential biological significance of these findings is discussed in this commentary in lieu of the entire process of reverse transcription.
- Subjects
REVERSE transcriptase; HIV; ENZYMES; DNA synthesis; NUCLEIC acids
- Publication
Viruses (1999-4915), 2011, Vol 3, Issue 4, p331
- ISSN
1999-4915
- Publication type
Opinion
- DOI
10.3390/v3040331