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- Title
Computational and Experimental Validation of B and T-Cell Epitopes of the <i>In Vivo</i> Immune Response to a Novel Malarial Antigen.
- Authors
Bergmann-Leitner, Elke S.; Chaudhury, Sidhartha; Steers, Nicholas J.; Sabato, Mark; Delvecchio, Vito; Wallqvist, Anders S.; Ockenhouse, Christian F.; Angov, Evelina
- Abstract
Vaccine development efforts will be guided by algorithms that predict immunogenic epitopes. Such prediction methods rely on classification-based algorithms that are trained against curated data sets of known B and T cell epitopes. It is unclear whether this empirical approach can be applied prospectively to predict epitopes associated with protective immunity for novel antigens. We present a comprehensive comparison of in silico B and T cell epitope predictions with in vivo validation using an previously uncharacterized malaria antigen, CelTOS. CelTOS has no known conserved structural elements with any known proteins, and thus is not represented in any epitope databases used to train prediction algorithms. This analysis represents a blind assessment of this approach in the context of a novel, immunologically relevant antigen. The limited accuracy of the tested algorithms to predict the in vivo immune responses emphasizes the need to improve their predictive capabilities for use as tools in vaccine design.
- Subjects
MALARIA; T cells; EPITOPES; IMMUNE response; ANTIGENS; VACCINES; HUMORAL immunity; ANTIGEN processing
- Publication
PLoS ONE, 2013, Vol 8, Issue 8, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0071610