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- Title
The Antioxidant and Antibacterial Activity of Moringa oleifera Extracts against some Foodborne Pathogens.
- Authors
Rajab Farhan, Shaymaa; AL-Azawi, Ahmed H.; AL-Shamary, Elham Ismaeel
- Abstract
The aim of this study is to determine the antioxidant and antibacterial activity of Moringa oleifera extracts. Maceration and Soxhlet apparatus were used to prepare aqueous and methanolic extracts respectively, while petroleum ether was used to extract seed oil. Many tests were conducted include, phytochemical detection, evaluation of antioxidant activity utilizing 2,2- diphenyl-1-picrylhydrazyl (DPPH) assay, total phenolic content. The extracts of Moringa oleifera were investigated against some foodborne pathogens include Staphylococcus aureus, Bacillus cereus, Escherichia coli and Klebsiella pneumonia. The results of phytochemical test showed the presence of alkaloids, phenols, favonoids, tannins, saponins and glycosides in aqueous and methanolic leaves extracts. The antioxidant activity showed that the aqueous and methanolic leaves extract was more effective than the seed's husk extract. The radical scavenging capacity (EC50) of methanolic and aqueous leaves extract were found to be (1.6 and 1.9 mg/ml) respectively, while the EC50 of methanolic and aqueous seed's husk were (5.1 and 10 mg/ml) respectively. The antibacterial activity of Moringa oleifera extracts showed the best effect was seen in the aqueous and methanolic leaves extract on Staph. aureus in 200 mg/ml with inhibition zone 14.83 and 22.6 mm respectively. The results of the minimum inhibitory concentration (MIC) showed that the methanolic leaves extract was 16 mg/ml for both Staph. aureus and E. coli, and 32 mg/ml for B. cereus and K. pneumonia, while the MIC of aqueous leaves extract was 64 mg/ml for all bacterial isolates.
- Subjects
MEDICINAL plants; ANTIOXIDANTS; PHYTOCHEMICALS; PLANT extracts; ANALYTICAL chemistry techniques; BACTERIA
- Publication
Medico-Legal Update, 2021, Vol 21, Issue 3, p486
- ISSN
0971-720X
- Publication type
Article
- DOI
10.37506/mlu.v21i3.3035