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- Title
Analysis of Genetic Diversity in Cymbidium Varieties Using SRAP.
- Authors
Pue Hee Park; Mi Seon Kim; Young Ran Lee; Pil Man Park; Dong Soo Lee; Byeong Woo Yae
- Abstract
Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened. Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between F1 varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.
- Subjects
CYMBIDIUM; CULTIVARS; GENETIC polymorphisms in plants; NUCLEOTIDE sequence; OPEN reading frames (Genetics); BRASSICA varieties; ACRYLAMIDE
- Publication
Korean Journal of Breeding Science, 2011, Vol 43, Issue 5, p399
- ISSN
0250-3360
- Publication type
Article