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- Title
Comparative Study of Captopril Derivatization Reaction by LC-UV, LC-MS and CE-UV Methods.
- Authors
Donáth-Nagy, Gabriella; Vancea, Szende; Imre, Silvia
- Abstract
The LC-UV, LC-MS and CE-UV study of chemical reaction between captopril and p-bromophenacyl bromide as derivatizing reagent is reported. During transformation of captopril, its thiol group is involved and the reaction is irreversible. Neutral or alkaline environments favor derivatization. The yield of reaction increases linear with the concentration of the reagent, while changes in temperature do not influence it significantly. Kinetic studies show that derivatization of captopril flows quickly, reaching a plateau after 30 minutes. Based on the study of these effects some important parameters such as derivatizing agent concentration, reaction time, temperature and pH were confined. The results were used for development of captopril determination methods both in pharmaceutical formulations and biological samples.
- Subjects
CAPTOPRIL; DERIVATIZATION; LIQUID chromatography-mass spectrometry; SULFHYDRYL group; TEMPERATURE; COMPARATIVE studies
- Publication
Croatica Chemica Acta, 2011, Vol 84, Issue 3, p423
- ISSN
0011-1643
- Publication type
Article
- DOI
10.5562/cca1787