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- Title
Differential Role of an Atypical Protein Kinase C, PKC ζ, in Regulation of Human Eosinophil and Neutrophil Functions.
- Authors
Kato, Masahiko; Yamaguchi, Takafumi; Tachibana, Atsushi; Kimura, Hirokazu
- Abstract
Background: Protein kinase C (PKC) comprises a family of isoenzymes playing a key role in downstream signaling and cell functions. PKCs are grouped according to molecular structure and mode of activation: ‘conventional’ PKCs (α, βI, βII, γ), ‘novel’ PKCs (δ, ε, μ, θ, η), and ‘atypical’ PKCs (ζ, τ/λ). Here we compared the influence of PKC ζ on the function of human eosinophils and neutrophils. Methods: After pretreating the cells with a myristoylated specific PKC ζ inhibitor, a myristoylated PKC η inhibitor, or bisindolylmaleimide I (Bis I; an inhibitor of conventional and novel PKCs), we examined N-formyl-methionyl-leucyl-phenylalanine (FMLP)- or 4-phorbol 12-myristate 13-acetate (PMA)-evoked superoxide anion (O2–) generation. Induced PKC translocation was characterized using confocal laser scanning microscopy. Results: The PKC ζ inhibitor significantly blocked FMLP- or PMA-induced O2– generation by eosinophils. However, this inhibitor attenuated PMA- but not FMLP-induced O2– generation by neutrophils. In contrast, Bis I inhibited FMLP-induced O2– generation by eosinophils and neutrophils in a similar manner. The PKC η inhibitor had no significant effect, since both cell types lack PKC η; this confirmed specificity of PKC ζ inhibitor effects. Finally, the translocation of PKC ζ to the plasma membrane induced by FMLP in both eosinophils and neutrophils was started at 1 min while the translocation was maintained for 15 min in eosinophils but not in neutrophils. Conclusion: An atypical PKC, PKC ζ, regulates human eosinophil and neutrophil functions in a differential manner. Copyright © 2005 S. Karger AG, Basel
- Subjects
EOSINOPHILS; NEUTROPHILS; PROTEIN kinases; PHOSPHOTRANSFERASES; ISOENZYMES; CELL physiology
- Publication
International Archives of Allergy & Immunology, 2005, Vol 137, p27
- ISSN
1018-2438
- Publication type
Article
- DOI
10.1159/000085429