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- Title
Functional Characterization of the Novel T599I-VKSRdel BRAF Mutation in a Follicular Variant Papillary Thyroid Carcinoma
- Authors
De Falco, Valentina; Giannini, Riccardo; Tamburrino, Anna; Ugolini, Clara; Lupi, Cristiana; Puxeddu, Efisio; Santoro, Massimo; Basolo, Fulvio
- Abstract
Context: Mutations in BRAF are rare in the follicular variant of papillary thyroid carcinoma (FV-PTC). Objective: We identified and functionally characterized a novel T599I-VKSR(600 – 603)del BRAF mutation in a FV-PTC patient. We analyzed in vitro the effects of this novel mutation in comparison with other thyroid cancer-associated mutations. Design: Expression vectors for the BRAF mutants were generated and their in vitro kinase activity, signaling along the MAPK pathway, and capability of stimulating transcription from an AP1- responsive reporter evaluated. Results: BRAF kinase and signaling were increased to a similar extent by the T599I-VKSR (600 – 603)del, V600E, and K601E mutations. Instead, the G474R, a mutation previously found in a FV-PTC, knocked down the BRAF kinase and its intracellular signaling. Some cancer-associated low-activity BRAF mutants stimulate the MAPK cascade via CRAF; however, the G474R protein lacked also this property. Conclusion: The T599I-VKSR(600 – 603)del is a novel gain-of-function mutation that targets BRAF in FV-PTC. Moreover, G474R is the first example of a mutation knocking down enzymatic BRAF activity in a FV-PTC. These findings underscore the importance of functional studies to characterize the role of BRAF mutations associated with thyroid cancer.
- Publication
Journal of Clinical Endocrinology & Metabolism, 2008, Vol 93, Issue 11, p4398
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/jc.2008-0887