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- Title
An ultramarine fluorescent protein with increased photostability and pH insensitivity.
- Authors
Tomosugi, Wataru; Matsuda, Tomoki; Tani, Tomomi; Nemoto, Tomomi; Kotera, Ippei; Saito, Kenta; Horikawa, Kazuki; Nagai, Takeharu
- Abstract
We report a pH-insensitive and photostable ultramarine fluorescent protein, Sirius, with an emission peak at 424 nm, the shortest emission wavelength among fluorescent proteins reported to date. The pH-insensitivity of Sirius allowed prolonged visualization of biological events in an acidic environment. Two fluorescence resonance energy transfer (FRET) pairs, Sirius-mseCFP and Sapphire-DsRed, allowed dual-FRET imaging with single-wavelength excitation, enabling detection of Ca2+ concentration and caspase-3 activation in the same apoptotic cells.
- Subjects
PROTEINS; WAVELENGTHS; ENERGY transfer; RESONANCE; CELLS; BIOMOLECULES
- Publication
Nature Methods, 2009, Vol 6, Issue 5, p351
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1317