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- Title
Identification of Nocardia farcinica by a PCR Primer Amplifying a Specific DNA Band for the Bacterium.
- Authors
Hasegawa, Taichi; Gonoi, Tohru; Ito, Junji; Kogure, Takahisa; Yazawa, Katsukiyo; Mikami, Yuzuru
- Abstract
A PCR primer specific to Nocardia farcinica was prepared based on sequence information random amplified polymorphic DNA (RAPD) analysis. The PCR primer amplifies N. farcinica species only; no amplification was observed in 25 other Nocardia strains that we tested. Specificity of the primer for N. farcinica was also confirmed using other fungal and bacterial strains that frequently isolated from clinical samples such as sputa and broncho alveolar lavage (VAL).
- Subjects
NOCARDIA; DNA; RAPD technique; BACTERIA; BRONCHOALVEOLAR lavage
- Publication
Japanese Journal of Medical Mycology, 2007, Vol 48, Issue 4, p173
- ISSN
0916-4804
- Publication type
Article
- DOI
10.3314/jjmm.48.173