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- Title
High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation.
- Authors
Anandapadamanaban, Madhanagopal; Andresen, Cecilia; Helander, Sara; Ohyama, Yoshifumi; Siponen, Marina I; Lundström, Patrik; Kokubo, Tetsuro; Ikura, Mitsuhiko; Moche, Martin; Sunnerhagen, Maria
- Abstract
The general transcription factor TFIID provides a regulatory platform for transcription initiation. Here we present the crystal structure (1.97 Å) and NMR analysis of yeast TAF1 N-terminal domains TAND1 and TAND2 bound to yeast TBP, together with mutational data. We find that yeast TAF1-TAND1, which in itself acts as a transcriptional activator, binds TBP's concave DNA-binding surface by presenting similar anchor residues to TBP as does Mot1 but from a distinct structural scaffold. Furthermore, we show how TAF1-TAND2 uses an aromatic and acidic anchoring pattern to bind a conserved TBP surface groove traversing the basic helix region, and we find highly similar TBP-binding motifs also presented by the structurally distinct TFIIA, Mot1 and Brf1 proteins. Our identification of these anchoring patterns, which can be easily disrupted or enhanced, provides insight into the competitive multiprotein TBP interplay critical to transcriptional regulation.
- Subjects
TATA box-binding protein; TATA box-binding protein-associated factors; YEAST; TRANSCRIPTION factors; CRYSTAL structure; NUCLEAR magnetic resonance; DNA-binding proteins; GENETIC mutation
- Publication
Nature Structural & Molecular Biology, 2013, Vol 20, Issue 8, p1008
- ISSN
1545-9993
- Publication type
Article
- DOI
10.1038/nsmb.2611