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- Title
Replication of Norovirus in Cell Culture Reveals a Tropism for Dendritic Cells and Macrophages.
- Authors
Wobus, Christiane E; Karst, Stephanie M; Thackray, Larissa B; Chang, Kyeong-Ok; Sosnovtsev, Stanislav V; Belliot, Gaël; Krug, Anne; Mackenzie, Jason M; Green, Kim Y; Virgin IV, Herbert W.
- Abstract
Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology. Noroviruses -- which cause epidemic gastroenteritis -- can now be grown in cells of the innate immune system, providing a tool to examine this pathogen while offering insights into norovirus biology.
- Subjects
DENDRITIC cells; NOROVIRUSES; INTRACELLULAR membranes; MACROPHAGES; TROPISMS; CELL culture; VIRAL tropism
- Publication
PLoS Biology, 2004, Vol 2, Issue 11, p1
- ISSN
1544-9173
- Publication type
Article
- DOI
10.1371/journal.pbio.0020432