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- Title
Engineering Short Peptide Sequences for Uranyl Binding.
- Authors
Lebrun, Colette; Starck, Matthieu; Gathu, Vicky; Chenavier, Yves; Delangle, Pascale
- Abstract
Peptides are interesting tools to rationalize uranyl-protein interactions, which are relevant to uranium toxicity in vivo. Structured cyclic peptide scaffolds were chosen as promising candidates to coordinate uranyl thanks to four amino acid side chains pre-oriented towards the dioxo cation equatorial plane. The binding of uranyl by a series of decapeptides has been investigated with complementary analytical and spectroscopic methods to determine the key parameters for the formation of stable uranyl-peptide complexes. The molar ellipticity of the uranyl complex at 195 nm is directly correlated to its stability, which demonstrates that the β-sheet structure is optimal for high stability in the peptide series. Cyclodecapeptides with four glutamate residues exhibit the highest affinities for uranyl with log KC=8.0-8.4 and, therefore, appear as good starting points for the design of high-affinity uranyl-chelating peptides.
- Subjects
AMINO acid sequence; AMINO acid analysis; METAL scaffolding; URANYL compounds synthesis; URANIUM compounds synthesis
- Publication
Chemistry - A European Journal, 2014, Vol 20, Issue 50, p16566
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201404546