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- Title
Efficient plant regeneration system for New Guinea Impatiens (Impatiens hawkeri W. Bull) CV. 'Violet' and 'Scarlet Bronze Leaf'.
- Authors
Zhang, Di; Wei, Jie; Zhou, Min; Li, Yang; Li, Xinyi; Wen, Yonghui; Huang, Meijuan; Huang, Haiquan
- Abstract
New Guinea Impatiens (Impatiens hawkeri W. Bull) is an eye-popping landscaping plant of bright and colorful blooms. A highly efficient in vitro plant regeneration system via direct shoot organogenesis was established for the first time from hypocotyl with partial cotyledons of New Guinea Impatiens. Our results showed that the explant sterilization method, basic medium type, AgNO3, sucrose and plant growth regulators have significantly influenced in vitro morphogenesis. The regeneration rate in regeneration media that MS supplemented with 0.5 mg·L−1TDZ and 0.1 mg·L−1NAA was acceptable, the induction rate of 'Violet' was 86.67%, and its proliferation coefficient was 5.27, while the induction rate of 'Scarlet Bronze Leaf' was 83.33%, and its proliferation coefficient 5.13. PIC could not induce clumped sprouts; however, it had a better effect on callus induction. We also included a shoot multiplication stage using regeneration New Guinea Impatiens medium that MS supplemented with 0.8 mg·L−16-BA, 0.5 mg·L−1TDZ and 0.05 mg·L−1NAA. Reducing sucrose concentration to 20 g·L−1 or adding 1 mg·L−1AgNO3 could alleviate the hyperhydricity phenomenon in the process of tufted bud proliferation. The optimal root culture medium for the regenerated seedlings of 'Violet' and 'scarlet bronze leaf' of New Guinea Impatiens was MS supplemented with 0.05 mg·L−1IBA, the rooting rate reached 100%.The study examined the micropropagation responses of New Guinea Impatiens in the presence of various growth regulators, which provide a simple and more suitable protocol adapted for the mass propagation of clones. Key message: A protocol for high-frequency shoot induction from hypocotyls with some cotyledons has been standardized for New Guinea Impatiens (Impatiens hawkeri W. Bull) CV.'Violet'and 'Scarlet Bronze Leaf'.
- Subjects
REGENERATION (Botany); IMPATIENS; PLANT regulators; BRONZE; GROWTH regulators; PLANT shoots
- Publication
Plant Cell, Tissue & Organ Culture, 2022, Vol 149, Issue 3, p549
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-022-02282-9