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- Title
Improved direct transformation via particle bombardment of split-immature embryo explants in soybean (Glycine max).
- Authors
Chennareddy, Siva; Cicak, Toby; Mall, Tejinder; Effinger, Katherine; Sardesai, Nagesh; Pareddy, Dayakar; Sarria, Rodrigo
- Abstract
An improved direct transformation method via particle bombardment of split-immature zygotic embryo explants with intact embryonic axis is reported. This method involves abiotic stress (cold treatment and plasmolysis) treatments of explants prepared from immature embryos of 8-10 mm size for improved somatic embryogeneis. Transgenic events were produced using optimized bombardment conditions and selection with hygromycin or glufosinate. Transgenic somatic embryos developed within as little as 4 weeks after bombardment of explants. Transgenic plants were regenerated 4-5 months after bombardment and the entire process from bombardment to T1 seed production took 7-9 months. Plants regenerated from the system were fertile and showed more than 90% heritability of the transgene to the next generation. Transformation frequencies of 5.4 and 2.7% (based on the number of bombarded split-immature embryo explants) were observed with hygromycin and glufosinate selection, respectively.
- Subjects
SOYBEAN; PLANT embryology; PLANT genetic transformation
- Publication
Plant Cell, Tissue & Organ Culture, 2018, Vol 135, Issue 1, p23
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-018-1440-7