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- Title
Muscular tubes of urethra engineered from adipose-derived stem cells and polyglycolic acid mesh in a bioreactor.
- Authors
Wang, Ying; Fu, Qiang; Zhao, Ren-Yan; Deng, Chen-Liang
- Abstract
We have explored the feasibility of using adipose-derived stem cells (ADSCs) and polyglycolic acid (PGA) for constructing muscular tubes of urethra in a bioreactor. With the induction of by 5-azacytidine, ADSCs were found to acquire a myoblast phenotype. Here we seeded ADSCs in a PGA mesh to construct the cell-PGA complex that was cultured statically for 1 week. Afterwards, the cell-PGA complex was subjected to extension stimulation in a bioreactor for 5 weeks. A muscular tube of urethra was formed after 6 weeks. Histological examination showed differentiated ADSCs and collagenous fibers had orientated well. This study demonstrates that tissue engineering of urethra tissues in vitro by using a bioreactor leads to tissue maturation and the differentiation of ADSCs. This novel technique could provide an effective approach for urethra tissue engineering.
- Subjects
STEM cell research; GLYCOLIC acid; URETHRA; BIOREACTOR research; TISSUE culture; TISSUE engineering
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 9, p1909
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1554-x