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- Title
Cellular aggregate size as the critical factor for flavonoid production by suspension cultures ofSaussurea medusa.
- Authors
Chun-xiang Fu; De-xiu Zhao; Yan Huang; Ma, Feng-shan
- Abstract
Three previously established cell lines (yellow, red and white) ofSaussurea medusawere investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75 ± 0.41 and 6.4 ± 0.31 mg l-1. Production of jaceosidin and hispidulin correlated with the sizes of compact callus aggregates (CCA) and cellular viability. In the red cell line, the sizes of CCA were predominantly of 2-4mm diameter and accounted for 64% biomass. This line had a sustained cell viability over 10 successive sub-cultures.
- Subjects
CELL aggregation; BIOMASS; SAUSSUREA; ASTERACEAE; CELL communication; BIOLOGY
- Publication
Biotechnology Letters, 2005, Vol 27, Issue 2, p91
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-004-6934-1