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- Title
Intracellular free calcium mediates glioma cell detachment and cytotoxicity after photodynamic therapy.
- Authors
Hong, Xin; Jiang, Feng; Kalkanis, Steven; Zhang, Zheng; Zhang, Xuepeng; Zheng, Xuguang; Jiang, Hao; Chopp, Michael; Kalkanis, Steven N; Zhang, Zheng Gang
- Abstract
Photofrin photodynamic therapy (PDT) caused a dose-dependent decrease of enzymatic cell detachment by trypsin/ethylenediamine tetra-acetic acid (EDTA) in human glioma U251n and U87 cells. This happened coincidently with the increase of intracellular free calcium ([Ca(2+)](i)). Thapsigargin, which increased [Ca(2+)](i), induced further decrease in enzymatic cell detachment and increased cytotoxicity. Opposite effects were observed when 1,2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetra-acetic acid tetrakis, an intracellular Ca(2+) chelator, was used. PDT-induced changes in [Ca(2+)](i) and cell detachment were not blocked by calcium channel antagonists nickel (Ni(2+)) or nimodipine, nor were they altered when cells were irradiated in a buffer free from Ca(2+) and magnesium (Mg(2+)), suggesting that [Ca(2+)](i) is derived from the internal calcium stores. Decreased cell migration was observed after PDT, as assessed by chemotactic and wound-healing assays. Our findings indicated that internal calcium store-derived [Ca(2+)](i) plays an important role in PDT-induced enzymatic cell detachment decrease and cytotoxicity. Cell migration may be affected by these changes.
- Subjects
PHOTOCHEMOTHERAPY; GLIOMAS; CALCIUM in the body; CELL-mediated cytotoxicity; CELL migration
- Publication
Lasers in Medical Science, 2009, Vol 24, Issue 5, p777
- ISSN
0268-8921
- Publication type
journal article
- DOI
10.1007/s10103-008-0640-5