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- Title
Effect of Mannitol on Helicobacter pylori-Induced Cyclooxygenase-2 Expression in Gastric Epithelial AGS Cells.
- Authors
Jeong Yeon Seo; Kyung Hwan Kim; Hyeyoung Kim
- Abstract
Oxygen radicals have been considered to be important regulatory molecules in Helicobacter pylori-induced gastric ulceration and carcinogenesis. H. pylori-induced inflammation has been shown to be associated with cyclooxygenase-2 (COX-2) expression in experimental animals and human patients. This study aimed to determine if H. pylori produces oxygen radicals and induces COX-2 expression in gastric epithelial cells. A further aim was to resolve whether or not the H. pylori-induced COX-2 expression could be inhibited by mannitol, a known hydroxyl radical scavenger, and superoxide dismutase (SOD), an antioxidant enzyme, which was used as a positive control. A human gastric epithelial cell line, AGS, treated with or without mannitol or SOD, was incubated in the presence or absence of H. pylori. mRNA expression and protein levels for COX-2 were determined by Northern blot and Western blot analysis, respectively. Levels of the COX-2 products, 6-keto-prostaglandin F[sub 1α] (6-keto-PGF[sub 1α] ) and thromboxane B[sub 2] (TXB[sub 2] ) and H[sub 2] O[sub 2] were measured in the medium. The results showed that H. pylori induced H[sub 2] O[sub 2] production, COX-2 mRNA and protein expression and increased the levels of 6-keto-PGF[sub 1α] and TXB[sub 2] . The H. pylori-induced COX-2 expression and the increase in the COX-2 products were inhibited by both mannitol and SOD. The inhibitory effect of mannitol on H. pylori-induced COX-2 expression suggests the possible involvement of oxygen radicals in the transcriptional regulation of the inflammatory mediators in gastric epithelial cells.Copyright © 2002 S. Karger AG, Basel
- Subjects
HELICOBACTER pylori; CYCLOOXYGENASE 2 inhibitors; THROMBOXANE synthesis; INFLAMMATORY mediators; EXFOLIATIVE cytology; REACTIVE oxygen species; CARCINOGENESIS; EPITHELIAL cells; PROSTANOIDS
- Publication
Pharmacology, 2002, Vol 66, Issue 4, p182
- ISSN
0031-7012
- Publication type
Article