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- Title
Interactions between interferon γ, tumour necrosis factor α, and interleukin-1 in modulating progesterone and oestradiol production by human luteinized granulosa cells in culture.
- Authors
Fukuoka, Masatsune; Yasuda, Keiko; Fujiwara, Hiroshi; Kanzaki, Hideharu; Mori, Takahide
- Abstract
We have reported that the cytokines, interleukin-1 (IL-1), tumour necrosis factor α (TNFα), and interferon (IFN)α, β, and γ modulate the steroidogenic function of human luteinized granulosa cells in culture. In the present study we examined the interactions between these cytokines in modulating progesterone and oestradiol production by these cells. Neither IL-1 nor TNFα had significant effects on human chorionic gonadotrophin (HCG)-stimulated progesterone production, whereas IFNγ (1–10 ng/ml) significantly reduced HCG-stimulated progesterone production by 26–37%. Concomitant treatment with IL-1 (1 ng/ml) did not further enhance the inhibitory effect of IFNγ on HCG-stimulated progesterone production. In contrast, the combination of TNFα (1 ng/ml) and IFNγ (10 ng/ml) acted synergistically to markedly inhibit HCG-stimulated progesterone production by 81%. In addition, IL-1 and TNFα, neither of which was effective alone, acted synergistically to reduce significantly HCG-stimulated progesterone production by 30%. The combination of TNFα and IFNγ also markedly inhibited follicle stimulating hormone (FSH)-stimulated oestradiol production by 97%, a significantly greater inhibition than that obtained with either cytokine alone. These results suggest that the cytokines may interact to modulate the steroidogenic function of luteal cells in the developing corpus luteum.
- Publication
Human Reproduction, 1992, Vol 7, Issue 10, p1361
- ISSN
0268-1161
- Publication type
Article