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- Title
Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica.
- Authors
Cibrián-Jaramillo, Angélica; Marler, Thomas E.; DeSalle, Rob; Brenner, Eric D.
- Abstract
We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.
- Subjects
MICROSATELLITE repeats; CYCADACEAE; SPECIES diversity; POPULATION genetics; GENOMES; HABITATS; WILDLIFE conservation; ENDANGERED species; SCIENTIFIC experimentation
- Publication
Conservation Genetics, 2008, Vol 9, Issue 4, p1051
- ISSN
1566-0621
- Publication type
Article
- DOI
10.1007/s10592-007-9447-3