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- Title
Multi-Gene mtDNA Primers for Use with Non-invasive Sampling of Asian Elephants.
- Authors
De, Rahul; Nigam, Parag; Williams, A. Christy; Goyal, Surendra P.
- Abstract
Understanding population genetic structure and phylogeography can be useful in framing conservation strategies for the Asian elephant. Non-invasive genetic sampling allows for such information to be obtained. However, amplifying long fragments from poor-quality faecal DNA is challenging. Therefore, we developed primers to amplify 270–474 bp fragments to cover ≈2 kb of the Asian elephant mtDNA genome across ATP8- ATP6, ND5 and D-loop regions. We obtained a 1978-bp sequence (in six fragments) from up to two-week-old faecal samples with a success rate of 79.8%. These primers would provide fine resolution phylogeography for the Asian elephant, and population-specific SNPs would aid in forensic tracking.
- Subjects
ASIATIC elephant; DNA primers; MITOCHONDRIAL DNA; PHYLOGEOGRAPHY
- Publication
Gajah, 2020, Issue 52, p24
- ISSN
1391-1996
- Publication type
Article