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- Title
Method evaluation and automation of bioMérieux kit detecting antibodies to Streptococcal DNAse B.
- Authors
Dobbs, Zaria; Tustin, Paul
- Abstract
Objectives: Detection of infection from Group A streptococcal bacteria (such as Streptococcus pyogenes) is crucial for preventing illness and later sequelae such as post-streptococcal glomerulonephritis and acute rheumatic fever. Infection is determined serologically, with a high enough titre of antibodies to either Streptolysin O or DNAse B indicating infection. The production of the Siemens Antibody Inhibition of Dnase B Enzyme kit has ceased, so an alternative is required to continue the ability to test. The selected option is the Anti-Streptodornase B (ASD) Antibody Testing kit manufactured by bioMérieux and used by Labtests in Auckland. Methods: A range of patient samples and RCPA samples that had been tested via the Siemens kit were collected over 2022, with age and clinical details recorded. Samples were run using the bioMérieux kit both manually and via the automated DSRie platform, then compared to the previous results on the Siemens kit. Results: The new bioMerieux kit had 100% specificity and 42% sensitivity compared to Siemens, and concordance was 66%. The kappa concordance was 0.37, which indicates a low level of agreement. A paired two tailed T test was performed; there was a significant difference between the interpretation of the kits (p<0.0003) and medium effect size (Cohen's d = 0.7425). Conclusion: There was inadequate comparability between the two kits. The bioMérieux kit was far less sensitive in comparison to the Siemens kit. When looking at just RCPA mean results, bioMérieux was concordant with 8/8 results, and Siemens was concordant with 7/8. While not comparable to the previous kit, bioMérieux is an acceptable alternative.
- Subjects
DIAGNOSTIC reagents &; test kits; STATISTICS; IMMUNOGLOBULINS; STREPTOCOCCAL diseases; INFECTION; STATISTICAL hypothesis testing; ESTERASES; SENSITIVITY &; specificity (Statistics); CHEMICAL inhibitors
- Publication
New Zealand Journal of Medical Laboratory Science, 2023, Vol 77, Issue 2, p95
- ISSN
1171-0195
- Publication type
Article