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- Title
Cloning, expression, crystallization and preliminary X-ray diffraction studies of staphylococcal superantigen-like protein 1 (SSL1).
- Authors
Dutta, Debabrata; Dutta, Anirudha; Bhattacharjee, Atanu; Basak, Amit; Das, Amit Kumar
- Abstract
Staphylococcus aureus produces a family of exotoxins which are structural homologues of superantigens and thus are called staphylococcal superantigen-like proteins (SSLs). Amongst the 14 SSL genes, ssl1 (SAOUHSC_00383) has been cloned in the pQE30 expression vector, overexpressed in Escherichia coli M15 (pREP4) cells and the protein purified to homogeneity. The protein was crystallized using 6% Tacsimate pH 6.0, 0.1 M MES pH 6.0, 25%( w/ v) polyethylene glycol 3350, 100 m M NDSB 256 at 298 K by the sitting-drop vapour-diffusion method. The crystals belonged to space group P21, with unit-cell parameters a = 77.9, b = 70.5, c = 126.5 Å, β = 106.2°. X-ray diffraction data were collected and processed to a maximum resolution of 2.5 Å. The crystal contains six molecules in the asymmetric unit.
- Subjects
STAPHYLOCOCCUS aureus infections; CLONING; CRYSTALLIZATION; X-ray diffraction; SUPERANTIGENS; EXOTOXIN
- Publication
Acta Crystallographica: Section F, Structural Biology Communications, 2014, Vol 70, Issue 5, p600
- ISSN
2053-230X
- Publication type
Article
- DOI
10.1107/S2053230X14006967