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- Title
Lactoferrin: A Novel Strategy for Antivenom Therapy.
- Authors
Elaraby, Abdel-Kader; El-Jakee, Jakeen; Fahmy, Aly; Kandil, Mai Mohamed; Mahmood, Zafar; Seida, Ahmed Adel
- Abstract
Snakebites worldwide represent a significant health hazard to humans and animals. Currently antivenom therapeutics is only the cure if given on proper time otherwise leads to death. The objective of this study was to evaluate the immunological effect of lactoferrin (Lf) in production of Naja nigricollis snake antibodies. Camel and cow milk whey were used as a source of 80 kDa Lf in a crude form and separated from milk whey using SDS-PAGE. This investigation was conducted through four experiments. In first experiment, we immunized of horses with N. nigricollis venom and camel milk whey; increased the anti-sera potency to (65 LD50) in comparison to the control group (45 LD50). For second experiment, we immunized of horses with the venom and cow milk whey which led to increase the anti-sera potency to (55.8 LD50) as compared to the control group (42.5 LD50). In the third experiment, using of standard bovine lactoferrin with the venom, the anti-sera potency increased to (75 LD50) as compared to the control group (50 LD50). In fourth experiment, we used of complete Freund's adjuvant with the venom; increase the anti-sera potency to (65 LD50) in compare to the control group (49.2 LD50). The immunological effect of lactoferrin was studied using Lymphocyte Transformation Test (LTT). The LTT mean values were 3.076±0.09 and 2.49±0.05 for the horses immunized with N. nigricollis venom / lactoferrin and the control group (horses injected with N. nigricollis venom without lactoferrin), respectively. This is the first study concluded that milk whey have the adjuvant effect on the antibodies levels and immunostimulatory effect in production of snake antisera from horses due to presence of lactoferrin. This specific property could be targeted as potential strategy for treatment of snake venom.
- Subjects
SNAKEBITE treatment; ANTIVENINS; LACTOFERRIN; NAJA nigricollis; IMMUNOGLOBULINS
- Publication
International Journal of Pharmaceutical Research & Allied Sciences, 2016, Vol 5, Issue 1, p50
- ISSN
2277-3657
- Publication type
Article