We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A Dendritic Cell-Activating Rv1876 Protein Elicits Mycobacterium Bovis BCG-Prime Effect via Th1-Immune Response.
- Authors
Choi, Seunga; Choi, Han-Gyu; Back, Yong Woo; Park, Hye-Soo; Lee, Kang-In; Gurmessa, Sintayehu Kebede; Pham, Thuy An; Kim, Hwa-Jung
- Abstract
The widely administered tuberculosis (TB) vaccine, Bacillus Calmette-Guerin (BCG), is the only licensed vaccine, but has highly variable efficiency against childhood and pulmonary TB. Therefore, the BCG prime-boost strategy is a rational solution for the development of new TB vaccines. Studies have shown that Mycobacterium tuberculosis (Mtb) culture filtrates contain proteins that have promising vaccine potential. In this study, Rv1876 bacterioferritin was identified from the culture filtrate fraction with strong immunoreactivity. Its immunobiological potential has not been reported previously. We found that recombinant Rv1876 protein induced dendritic cells' (DCs) maturation by MAPK and NF-κB signaling activation, induced a T helper type 1 cell-immune response, and expanded the population of the effector/memory T cell. Boosting BCG with Rv1876 protein enhanced the BCG-primed Th1 immune response and reduced the bacterial load in the lung compared to those of BCG alone. Thus, Rv1876 is a good target for the prime-boost strategy.
- Subjects
T cells; MYCOBACTERIUM tuberculosis; TUBERCULOSIS; MYCOBACTERIUM bovis; RECOMBINANT proteins; DENDRITIC cells; LUNGS; PROTEINS; IMMUNE response
- Publication
Biomolecules (2218-273X), 2021, Vol 11, Issue 9, p1306
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom11091306