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- Title
HvGBSSI mutation at the splicing receptor site affected RNA splicing and decreased amylose content in barley.
- Authors
Xiuqin Feng; Rahman, Md. Mostafijur; Qian Hu; Bang Wang; Hassan Karim; Guzmán, Carlos; Harwood, Wendy; Qiang Xu; Yazhou Zhang; Huaping Tang; Yunfeng Jiang; Pengfei Qi; Mei Deng; Jian Ma; Jingyu Lan; Jirui Wang; Guoyue Chen; Xiujin Lan; Yuming Wei; Youliang Zheng
- Abstract
Granule-bound starch synthase I (HvGBSSI) is encoded by the barley waxy (Wx-1) gene and is the sole enzyme in the synthesis of amylose. Here, a Wx-1 mutant was identified from an ethyl methane sulfonate (EMS)-mutagenized barley population. There were two single-base mutations G1086A and A2424G in Wx-1 in the mutant (M2-1105). The G1086A mutation is located at the 3' splicing receptor (AG) site of the fourth intron, resulting in an abnormal RNA splicing. The A2424G mutation was a synonymous mutation in the ninth intron. The pre-mRNA of Wx-1 was incorrectly spliced and transcribed into two abnormal transcripts. The type I transcript had a 6 bp deletion in the 5' of fifth exon, leading to a translated HvGBSSI protein lacking two amino acids with a decreased starch-binding capacity. In the type II transcript, the fourth intron was incorrectly cleaved and retained, resulting in the premature termination of the barley Wx-1 gene. The mutations in the Wx-1 decreased the enzymatic activity of the HvGBSSI enzyme and resulted in a decreased level in amylose content. This work sheds light on a new Wx-1 gene inaction mechanism.
- Subjects
RNA splicing; BARLEY; AMYLOSE; AMINO acids; STARCH
- Publication
Frontiers in Plant Science, 2022, Vol 13, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2022.1003333