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- Title
Demonstration of seed inoculation of dimer infectious clones of mungbean yellow mosaic India virus-soybean isolate.
- Authors
Gupta, Taruna; Singhal, Tripti; Dhir, Sunny; Chandel, Vanita; Rishi, Narayan; Raj, S. K.; Srivastava, Ashish
- Abstract
In this study, the full-length components of mungbean yellow mosaic India virus (MYMIV) DNA-A (MW590720 & MW600934) and DNA-B (MW659819 & MW659820) from a soybean isolate were cloned and sequenced. Nucleotide sequence analysis of both MYMIV components revealed > 96% identity and close ancestry with MYMIV isolates from legumes in Southeast Asia. Furthermore, dimeric infectious clones of MYMIV were generated in the pCAMBIA1302 vector, and a seed infiltration protocol was established for mungbean, soybean, and Nicotiana tabacum. Agroinfiltration induced yellow mosaic symptoms in mungbean and N. tabacum plants 3 weeks post-infiltration, which were further confirmed by PCR using MYMIV-specific DNA primers.
- Subjects
SOUTHEAST Asia; VIRUS cloning; MOSAIC viruses; NUCLEOTIDE sequencing; DNA primers; SEEDS; MUNG bean; VACCINATION
- Publication
3 Biotech, 2023, Vol 13, Issue 11, p1
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-023-03778-7