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- Title
Effects of GH on lipid peroxidation and neutrophil superoxide anion-generating capacity in hypopituitary adults with GH deficiency.
- Authors
Smith, J. C; Lang, D; McEneny, J; Evans, L. M; Scanlon, M. F; Young, I; Davies, J
- Abstract
Summary objectives Untreated growth hormone deficiency (GHD) is implicated in the increased cardiovascular risk associated with adult hypopituitarism. Oxidative stress, predisposing to lipid peroxidation, may be an important mediator of endothelial dysfunction, a pro-atherogenic state associated with adult GHD. design and patients In a randomized, double-blind, placebo-controlled study we investigated the effects of GH replacement on low-density lipoprotein (LDL) oxidation and neutrophil superoxide (O2- ) generating capacity in 32 GHD adults (19 males, 13 females; age range 19–64 years) over 3 months. Thirty age- and sex-matched healthy controls were also studied. measurements Lipid hydroperoxides (HPOs) in plasma were measured using the ferrous oxidation with xylenol orange (FOX) assay. The susceptibility of LDL to oxidation was assessed by the copper-catalysed lag phase of LDL oxidation. Neutrophil O2- generating capacity was assessed by a lucigenin-based chemiluminescent assay of NADPH oxidase activity. Body composition was assessed using bioelectrical impedance analysis. results Compared to controls, GHD subjects had higher LDL cholesterol (4·0 ± 0·8 vs. 3·5 ± 0·9 mmol/l, P < 0·01) and higher triglyceride concentrations (2·3 ± 1·5 vs. 1·1 ± 0·7 mmol/l, P < 0·001) but lower HDL cholesterol (1·1 ± 0·3 mmol/l vs. 1·4 ± 0·4 mmol/l, P < 0·01), lower levels of HPOs (0·72 ± 0·35 vs. 0·92 ± 0·20 µm, P < 0·01) and lower basal (2·5 ± 1·5 vs. 4·5 ± 2·3 mV/5 × 105 neutrophils, P < 0·01) and peak post-activation levels (23·2 ± 11·1 vs. 34·4 ± 15·6 mV/5 × 105 neutrophils, P < 0·01) of neutrophil O2- generation. GH replacement resulted in an increase in...
- Subjects
SOMATOTROPIN; LIPIDS; PITUITARY dwarfism
- Publication
Clinical Endocrinology, 2002, Vol 56, Issue 4, p449
- ISSN
0300-0664
- Publication type
Article
- DOI
10.1046/j.1365-2265.2002.01493.x