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- Title
c-Jun as a one-way valve at the naive to primed interface.
- Authors
Li, Dongwei; Luo, Ling; Guo, Lin; Wu, Chuman; Zhang, Ran; Peng, Yuling; Wu, Menghua; Kuang, Junqi; Li, Yan; Zhang, Yudan; Xie, Jun; Xie, Wenxiu; Mao, Rui; Ma, Gang; Fu, Xiuling; Chen, Jiekai; Hutchins, Andrew P.; Pei, Duanqing
- Abstract
Background: c-Jun is a proto-oncogene functioning as a transcription factor to activate gene expression under many physiological and pathological conditions, particularly in somatic cells. However, its role in early embryonic development remains unknown. Results: Here, we show that c-Jun acts as a one-way valve to preserve the primed state and impair reversion to the naïve state. c-Jun is induced during the naive to primed transition, and it works to stabilize the chromatin structure and inhibit the reverse transition. Loss of c-Jun has surprisingly little effect on the naïve to primed transition, and no phenotypic effect on primed cells, however, in primed cells the loss of c-Jun leads to a failure to correctly close naïve-specific enhancers. When the primed cells are induced to reprogram to a naïve state, these enhancers are more rapidly activated when c-Jun is lost or impaired, and the conversion is more efficient. Conclusions: The results of this study indicate that c-Jun can function as a chromatin stabilizer in primed EpiSCs, to maintain the epigenetic cell type state and act as a one-way valve for cell fate conversions.
- Subjects
CHECK valves; SOMATIC cells; GENE expression; TRANSCRIPTION factors; EMBRYOLOGY
- Publication
Cell & Bioscience, 2023, Vol 13, Issue 1, p1
- ISSN
2045-3701
- Publication type
Article
- DOI
10.1186/s13578-023-01141-0