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- Title
The Effect of Chitosan and Acrylate Acid Complex into Acrylic Resin as Denture Material Against Fibroblast and Inflammatory Cells.
- Authors
Ismiyati, Titik; Alhasyimi, Ananto Ali; Siswomihardjo, Widowati; Supriatno
- Abstract
This study investigates the effect of chitosan and acrylate acid addition in the acrylic resin as denture material toward the number of fibroblasts and inflammatory cells including macrophages, lymphocytes, and plasma cells. This study is an experimental laboratory research. Nine Wistar rats (Rattus Novergicus) were divided into 3 groups (n= 3). Control group receiving treatment without mixture of acrylic resin (CO), group 2 acrylic resin mixture with chitosan and 1% acrylate acid (ARC1), while group 3 acrylic resin mixture with chitosan and acrylate acid 2% (ARC2). The acrylic resin plate is formed in 4 mm-sized discs with 2 mm thickness. Resin samples were implanted into subcutaneous dorsal-area of Wistar rats, then stitched. Microscopic evaluation conducted on day 7 post-implantation. Histological examination combined hematoxylin eosin staining and 400x magnification light microscope. Fibroblasts, macrophages, lymphocytes and plasma cells were counted using the Image J program. Data were analyzed using one-way ANOVA and followed by LSD test with 95% level of significance. Results revealed that fibroblasts, macrophages, lymphocytes and plasma cells count in the CO group were higher than the ARC1 and ARC2 groups. Greater concentration of chitosan and acrylate acid in the acrylic resin decreased the mean of fibroblasts, macrophages, lymphocyte and plasma cells. One-way ANOVA test presented significant differences in the fibroblasts, macrophages, lymphocytes and plasma cells count of the control and treatment groups (p<0.05). The LSD test indicated significant difference between CO and ARC2 group. We concluded that the combination of acrylic resin with chitosan and 2% acrylate acid concentration is properly perceived by rat's body tissue.
- Subjects
ACRYLIC resins; ACRYLIC acid; FIBROBLASTS; CHITOSAN; PLASMA cells; DENTURES; PLASMA cell diseases
- Publication
Journal of International Dental & Medical Research, 2021, Vol 14, Issue 4, p1459
- ISSN
1309-100X
- Publication type
Article