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- Title
The utility of flow cytometry for the detection of tumor cells in cerebrospinal fluid of patients with acute leukemia.
- Authors
Torres, Ximena; De Los Reyes, Iliana; Vizcaíno, Martha; Carolina Guzmán, Paula; Campos, Alba; Peñalosa, Niyireth; Uribe, Ana María; Saavedra, Carlos; Romero, Martha; Cuéllar, Gina; Martin, Liliana; Rodriguez, Paula; Cardozo, Claudia; Quijano, Sandra
- Abstract
Central nervous system infiltration by acute leukemia is a poor prognosis variable, and conventional cytology is the gold standard for its diagnosis; the technique is highly specific but not sensitive. To improve the diagnosis, flow cytometry has been used in different studies, showing greater sensitivity in the detection of leukemic cells. This study aimed to evaluate the presence of tumor cells by flow cytometry and conventional cytology, in cerebrospinal fluid from patients with acute leukemia as well as its relationship with clinical and biological parameters. In total, 156 CSF samples from 55 children with acute leukemia were studied. We found the following results: FCM-/CC- 131=156; FCMC+/CC- 19=156; FCM-+/CC+ 0; FCMC+/CC+ 1/156; FCM-+/CC suspicious 1=156; and FCMC+/CC suspicious 4=156. Patients with B-cell acute lymphoblastic leukemia and FCMC showed a lower response to steroid-treatment, abnormal karyotype, neurological symptoms, and worse relapsefree survival. Patients with T-cell acute lymphoblastic leukemia and FCMC demonstrated association with thrombocytopenia. In conclusion, flow cytometry has greater sensitivity for the detection of tumor infiltration in cerebrospinal fluid, a finding that correlates with prognostic parameters in patients with acute leukemia.
- Subjects
ACUTE leukemia; FLOW cytometry; CEREBROSPINAL fluid examination; CEREBROSPINAL fluid; CYTOLOGICAL techniques; CYTOLOGY; LYMPHOBLASTIC leukemia; CENTRAL nervous system
- Publication
Universitas Scientiarum, 2022, Vol 27, Issue 1, p15
- ISSN
0122-7483
- Publication type
Article
- DOI
10.11144/Javeriana.SC271.tuof