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- Title
Initial cytotoxicity assays of media for sulfate-reducing bacteria: An endodontic biopharmaceutical product under development.
- Authors
HEGGENDORN, Fabiano Luiz; de Carvalho SILVA, Gabriela Cristina; CARDOSO, Elisama Azevedo; CASTRO, Helena Carla; GONÇALVES, Lúcio Souza; DIAS, Eliane Pedra; de Oliveira Freitas LIONE, Viviane; LUTTERBACH, Márcia Teresa Soares
- Abstract
This study assessed the cell viability of the inoculation vehicle of BACCOR (a combination of sulfate-reducing bacteria plus a culture media for bacteria), a biopharmaceutical product under development for dental use as aid in fractured endodontic file removal from the root canal. Different culture media for bacteria were evaluated: modified Postgate E (MCP-E mod), Modified Postgate E without Agar-agar (MCP-E w/Ag), Postgate C with Agar-agar (MCP-C Ag) and Postgate C without Agar-agar (MCP-C w/Ag). Cytotoxicity was quantified by the MTT test, exposing L929 and Vero cell lines to the vehicles over 24 h. The exposure of L929 cell line to MCP-E w/Ag resulted in biocompatibility (52% cell viability), while the exposure of the Vero kidney line revealed only MCP-E mod as cytotoxic. When diluted, all the vehicles showed biocompatibility with both cell lines. MCP-E w/Ag was the vehicle chosen for BACCOR, because of its biocompatibility with the cells used.
- Subjects
CELL-mediated cytotoxicity; ANTIBIOTICS; BACTERIOPHAGES; BIOPHARMACEUTICS; CELL lines
- Publication
Dental Materials Journal, 2016, Vol 35, Issue 5, p762
- ISSN
0287-4547
- Publication type
Article
- DOI
10.4012/dmj.2015-360